Method Development and Simultaneous Estimation for Skeletal Muscle Relaxant in Solid Dosage Form Using Rp-hplc
Year: 2026
Type: article
Abstract: A reversed-phase liquid chromatography (RP-HPLC) method was developed for the simultaneous determination of tolperisone hydrochloride (TOLP) and etodolac (ETD) in a solid dosage form. The analysis was performed using a Grace C18 column. The optimal composition of the mobile phase was found to be Methanol: Buffer 55:45 (v/v). The flow rate was set at 1.4 ml/min and UV detection was carried out at 254 nm. Retention times of Etodolac 8.30 and Tolperisone (9.36). The method showed a good linearity range between 10-50ug/ml for etodolac and 2-10 ug/ml for tolpresione. The LOD was found to be 0.17µg/mL and 0.08µg/mL for Etodolac and Tolperisone respectively and LOQ was found to be 0.51µg/mL and 0.25µg/mL for Etodolac and Tolperisone respectively. % Recovery study for Etodolac and Tolpresione was found to be 100.57,99.94 respectively. The RSD for the precision of the method was found to be less than 2%.
Author: *Ms. Shraddha Lunge
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7165017035
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