Figure S1 from Synergistic Treatment of Prostate Cancer and Multiple Myeloma using CD46-Targeted Radioimmunotherapy Antibody–Drug Conjugates
Year: 2026
Type: other
Abstract: Figure S1: MALDI mass spectrometry analysis of YS5-MMAE and Macropa-PEG4-YS5-MMAE revealed an average conjugation of 0.91 Macropa-PEG4 moieties per YS5-MMAE molecule. The disulfide bond reduction process used for MMAE conjugation led to the appearance of multiple peaks in the YS5-MMAE spectrum. For quantifying Macropa-PEG4 conjugation, the doubly charged peak at 75 kDa was used. The difference in peak intensity between YS5-MMAE and Macropa-PEG4-YS5-MMAE was divided by the molecular weight of Macropa-PEG4 to estimate the degree of conjugation.
Authors: Anil P. Bidkar, Scott Bidlingmaier, Anju Wadhwa, Ellis Mayne, Athira Raveendran, Shubhankar Naik, Cheng Xue, Megha Basak, Kondapa Naidu Bobba, Bonell Patiño-Escobar, Nancy Greenland, Juan Antonio Camara Serrano, Veronica Steri, Jonathan Chou, Arun Wiita, Jiang He, David M. Wilson, Rahul Aggarwal, Henry F. VanBrocklin, Youngho Seo, Bin Liu, Robert R. Flavell
Language: English
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7165017387