Protocol for total internal reflection fluorescence microscopy image preprocessing and radial analysis of LFA-1 in T cell immune synapses
Year: 2026
Type: article
Abstract: Here, we present a protocol for total internal reflection fluorescence (TIRF) microscopy image preprocessing and radial analysis of lymphocyte function-associated antigen-1 (LFA-1) in T cell immune synapses. We describe steps for combining customizable Fiji/ImageJ macros for image background subtraction and segmentation with flexible R-based scripts for peripheral supramolecular activation cluster (pSMAC) center identification, center-based cropping, and radial analysis of synaptic LFA-1 in T cells. This workflow supports established analyses, including radial averaging/spinning and fluorescence intensity measurements across the synapse.
Source: STAR Protocols
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,550
Awards: G1132377918, Who’s got the plasmid? Tracking the spread of antimicrobial resistance genes in the human gut microbiome at the single-cell level, G1953439041, G1960764132, G1984984145, G2572405796, Trovärdig screen för 4e generationens CAR T-cellsutveckling, G4613856683, G5095397854, G712881263, Intracellular transport of Integrins, G8392682762, Förbättring av pankreascancerdiagnostik genom mätning av telomerlängd hos individuella celler
OpenAlex ID: W7165032041