Figure S5 from Dynamics of Immune Reconstitution and Impact on Outcomes across CAR-T Cell Products in Large B-cell Lymphoma
Year: 2026
Type: other
Abstract: Supplementary Figure 5. Gating strategy for the TBNK assay. (A) FSC vs SSC dot plot was used to identify debris, characterized as low SSC and low FSC. Non-debris events are used for subsequent gating. (B) Using a FSC-A vs FSC-H dot plot, a singlet gate isused to include singlet and remove doublet events. (C) Singlet events are used in a CD45 vs SSC dot plot, and a gate is set around the CD45+ lymphocyte events, characterized by low SSC and bright CD45. (D) CD45+ lymphocyte events arethen passed through a CD3 vs CD19 dot plot to identify CD19+ B cells and CD3+ T cells. (E) CD3+ T cells are further identified as CD3+CD4+ T helper cells and CD3+CD8+ T cytotoxic cells in a CD4 vs CD8 dot plot. (F) Finally, CD56+CD16+ NK cells are identified in a CD3 vs CD16+56 dot plot.
Authors: Danny Luan, Susan DeWolf, Teng Fei, Sandeep Raj, Gunjan L. Shah, Caleb A. Lareau, Mohammad Alhomoud, Gilles Salles, Alfredo Rivas-Delgado, Kai Rejeski, Jae H. Park, Efrat Luttwak, Alejandro Luna de Abia, Magdalena Corona, Evangelos Ntrivalas, Giulio Cassanello, Marina Gomez-Llobell, Allison Parascondola, Michael Scordo, Katharine C. Hsu, M. Lia Palomba, Miguel-Angel Perales, Roni Shouval
Topic: CAR-T cell therapy research
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7165035342
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