Supplemental Figure S3 from VAX014 Activates Tumor-Intrinsic STING and RIG-I to Promote the Development of Antitumor Immunity
Year: 2026
Type: supplementary-materials
Abstract: Supplemental Figure S3. VAX014 does not activate endosomal TLR3 in MB49 cells. MB49 or J774A.1 cells were plated (5.0 × 105 cells/well) and treated with the TLR3 agonist Poly(I:C) (0.65 μg/mL), VAX-I or VAX014 rBMCs for ∼20 hours. A) IFN-β in supernatants of J774A.1 cells measured via ELISA (n = 3). B) Surface expression of PD-L1 and MHC-I in MB49 cells co-incubated with the supernatants from treated J774A.1 cells, as determined by flow cytometry (n = 3–6). C) IFN-β in supernatants of MB49 cells (n = 3). D) Surface expression of PD-L1 and MHC-I in MB49 cells as measured by flow cytometry (n = 6). Histograms depict mean ± SEM of all combined experiments and the dashed lines represent baseline expression of the untreated control for each cell type.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7165056519