Supplemental Figure S13 from VAX014 Activates Tumor-Intrinsic STING and RIG-I to Promote the Development of Antitumor Immunity
Year: 2026
Type: supplementary-materials
Abstract: Supplemental Figure S13. In vitro characterization of all MB49 RIG-I KO clones. A-D) MB49WT (WT) and MB49RIG-I KO (RIG-I KO) cells were plated (5.0x105 cells/well) and treated with DMXAA (30 μg/mL) or VAX014 (MOI 300) ± H-151 (4 μg/mL) for ∼20 hours. A) IFN-β in supernatants of treated cells as measured via ELISA (n= 3-6). B) Ratio of pIRF3 to IRF3 in cells 4 hours post-treatment analyzed via Western blot. Histogram includes values from two independent experiments. C) Representative Western blot for data in B). D) Surface expression of PD-L1 and MHC-I in cells as determined by flow cytometry (n= 5). Histograms depict mean ± SEM of all combined experiments and dashed lines represent the baseline expression value of the corresponding untreated control. #indicates which RIG-I KO clone is represented in Figure 4.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7165057820