Figure 2 from Metastasis-Associated Wound Repair Promotes Reciprocal Lung Epithelium Activation and Breast Cancer Metastatic Outgrowth
Year: 2026
Type: other
Abstract: Chronic wound repair in the lung surrounding growing metastases. A, MMTV-PyMT metastatic lungs were H&E- and IHC-stained for cell-specific markers of lung wound repair (n > 50 metastases from 2–7 mice per stain; see Supplementary Table S1A for sample number details). Shown are representative images from metastases of different sizes, with black arrows indicating small metastases; scale bar, 100 μm. The percentage of positively stained cells, normalized to the total number of cells, was quantified in the 100 μm surrounding metastases. Mean ± SEM (unpaired t tests with Welch correction); *, P ≤ 0.05; ***, P < 0.001. B, Multi-IF staining for a lung wound repair cell signature in MMTV-PyMT and Met-1 metastatic lungs. Shown are representative images from MMTV-PyMT metastases of different sizes; scale bars, 25 μm, inset zoom 3×. The wound repair score, calculated as the total number of wound repair–related cells in the 300 μm surrounding metastases from the spontaneous MMTV-PyMT metastasis model (n = 16 metastases from 2–4 mice; unpaired t tests with Welch correction) and the late-stage Met-1 intravenous (IV) metastasis model (n = 50 metastases from five mice; unpaired t tests). Mean ± SEM. C, MMTV-PyMT metastatic lungs were stained for cell-specific activation markers. Shown are representative images from metastases of different sizes, with black arrows indicating small metastases. For polarized light picrosirius red images, metastases are outlined in white. BV, blood vessel; scale bar, 100 μm, inset zoom 4×. D, Multi-IF staining of MMTV-PyMT metastatic lungs for AT2 cell activation. Shown are representative images from metastases of different sizes, with white arrows indicating proSP-C and Ki67 double-positive proliferating AT2 cells; scale bars, 25 μm, inset zoom 3×. The percentage of proSP-C–positive cells that are Ki67-positive was quantified in the lung surrounding metastases: <300 μm surrounding metastases for adjacent cells and >300 μm for nonadjacent cells (n = 17 metastases from 3–5 mice; unpaired t tests); mean ± SEM. E, Cytokine array performed on lungs from mice with a low or high metastatic burden using the late-stage Met-1 IV metastasis model (n = 3 mice per group; multiple unpaired t tests with Welch correction). Mean ± SEM; *, P ≤ 0.05. S, small; M, medium; L, large metastases.
Topic: Cancer Cells and Metastasis
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7165061997
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