Work

Identification and Characterization of the Detoxification Genes from the Transcriptome of Plagiodera versicolora

Year: 2026

Type: article

Abstract: Plagiodera versicolora (Coleoptera: Chrysomelidae), the willow leaf beetle, is a leaf-eating pest that generally occurs on salicaceous trees. However, there is a blank of identification and phylogenetic relationship of the detoxification genes in P. versicolora. Here, we identified four detoxification gene families (glutathione S-transferases: GSTs, UDP-glycosyltransferases: UGTs, cytochrome P450 monooxygenases: CYPs and carboxylesterases: COEs) from the adult antennal transcriptome data. In all, 146 candidate detoxification genes including 22 GSTs, 20 UGTs, 60 CYPs, and 44 COEs were identified. We used quantitative real-time PCR technology to explore the tissue expression patterns of 12 PvGSTs in P. versicolora. The results showed that 7 PvGSTs have significantly high expression in antennae, indicating these PvGSTs may play an important role in degrade and/or inactivate the sex pheromones and host volatiles. The identification and phylogenetics of the detoxification genes in P. versicolora extended the database in Coleoptera and contributed to the subsequent in-depth research for function about detoxification genes.

Source: Insects

DOI: 10.3390/insects17060643

Authors: Xiao-Long Liu, Hai-Dong Sun, Yi-Wen Pei, Min Lu, Hai-Nan Zhang

Institutions: State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Yangzhou University

Language: English

Topic: Insect Resistance and Genetics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $3,137

Funders: National Natural Science Foundation of China, China Postdoctoral Science Foundation