Additional file 1 of Therapeutic targeting of RAGE/STAT3 signaling abrogates S100A7-driven breast tumorigenicity and immune suppression
Year: 2026
Type: other
Abstract: Supplemental Figure 1: Analysis of S100A7 downregulation on Stat3 phosphorylation and effect of alone or combinatorial treatment of Stat3 and RAGE inhibitors on cell viability of S100A7 expressing TNBC cells. (A). The cell lysates were harvested from Scramble (Scr) control and S100A7 knockdown (KD) MDA-MB-468 cells generated using an independent shRNA clone 2 and were analyzed for the level of S100A7, phosphorylation of activated phospho-Stat3 (Ser727 and Tyr705), total Stat3, and GAPDH. Cell viability assays of B & C). S100A7 overexpressing MDA-MB-231 and (D & E). S100A7-expressing MDA-MB-468 cells treated with vehicle controls (VC) or different concentrations of Stat3 and RAGE inhibitors. Cell viability assays of (F). S100A7 overexpressing MDA-MB-231 and (G). S100A7 expressing MDA-MB-468 cells treated with vehicle controls (VC) or combinations of different concentrations of Stat3 and RAGE inhibitors. Data are mean±SEM. (n= 3).
Source: Figshare
Institution: The Ohio State University
Language: English
Topic: S100 Proteins and Annexins
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7165363121