Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions
Year: 2026
Type: article
Abstract: Lipopolysaccharides (LPS) are a key component in the defensive barrier of the outer membrane (OM) of Gram-negative bacteria; they are a major drug target and bacteriophage access point. Biomimetic platforms presenting LPS are therefore important to study OM biophysics of pathogens and initial bacteriophage infection steps. Herein, we present robust protocols for the preparation of LPS-containing supported lipid bilayers (SLBs) incorporating either Salmonella LPS or OM vesicles (OMVs). We characterized SLBs using quartz crystal microbalance with dissipation (QCM-D) and fluorescence microscopy. We probed their interactions with O-antigen specific Salmonella phages and their tailspike receptor binding proteins. Altogether, this work provides a roadmap to create versatile LPS-based platforms that facilitate studies of interactions between phages and Gram-negative bacterial membranes.
Source: FEBS Letters
Language: English
Subfield: Ecology
Field: Environmental Science
Domain: Physical Sciences
Open Access status: hybrid
Estimated APC paid: $3,980
Awards: G2252777492, G2630566782, G4103379771, The role of cell-surface glycosaminoglycans in modulating virus binding, diffusion, and internalization on the cell membrane: a study of Herpes Simplex Virus, Glycan Hopscotch: coronavirus’ dynamic journey from animals to humans, Filovirus interactions on the cell surface: finding the sweet spots, Glycan hopscotch: decoding how adenoviruses hop, skip, and jump their way into the cell
OpenAlex ID: W7165782008