The M91 metallopeptidase domain of the T6SS effector protein SED_RS06335 encoded in Salmonella Dublin SPI-19 exhibits antibacterial activity
Year: 2026
Type: article
Abstract: Objective The Type VI Secretion System (T6SS) is a contractile apparatus made of several proteins playing a significant role in the fitness and virulence of many Gram-negative bacteria. The SPI-19 T6SS gene cluster is a virulence factor of Salmonella Dublin contributing to host colonization and antibacterial activity. Previously, we demonstrated that SED_RS06335 (an Rhs protein with a C-terminal M91 domain) is responsible for antibacterial activity of the T6SS SPI−19 ; however, the role of the M91 domain in this phenotype is unknown. Thus, the objective of this study is to provide experimental evidence that the antibacterial activity of SED_RS06335 effector is attributable to its C-terminal M91 domain. Results Here, we determine through interbacterial competition and heterologous expression assays that M91 domain of SED_RS06335 displays antibacterial activity. Furthermore, a three-dimensional structural model of M91 domain revealed a high similarity with the metallopeptidase neurotoxin type A (BotA) from Clostridium botulinum . Interestingly, our results suggest that the M91 domain is responsible for the antibacterial activity of the SED_RS06335 effector in Salmonella Dublin.
Source: BMC Research Notes
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,690
Awards: G4363455504, FUNCTIONAL AND STRUCTURAL CHARACTERIZATION OF TYPE VI SECRETION SYSTEM SUBUNITS AND EFFECTORS OF THE FOODBORNE PATHOGEN CAMPYLOBACTER JEJUNI, MOLECULAR MECHANISMS OF SALMONELLA T3SS EFFECTOR PROTEINS THAT SUBVERT INTRACELLULAR TRAFFICKING PATHWAYS IN THE SOCIAL AMOEBA DICTYOSTELIUM DISCOIDEUM, FUNCTIONAL AND BIOCHEMICAL CHARACTERIZATION OF A PUTATIVE ANTIBACTERIAL TYPE VI SECRETION SYSTEM EFFECTOR OF THE FOODBORNE PATHOGEN SALMONELLA DUBLIN, COORDINATED ACTIVITY OF TWO CONTACT-DEPENDENT MOLECULAR WEAPONS BY A NOVEL C-DI-GMP PHOSPHODIESTERASE IN VIBRIO PARAHAEMOLYTICUS, G812293698
OpenAlex ID: W7165845188