Work

Protocol for genomic mapping of chromatin targets using high-throughput CUT&RUN

Year: 2026

Type: article

Abstract: The genomic distribution of proteins that define chromatin state, including transcription factors, chromatin modifiers, and histone modifications, is critical for understanding gene regulation, cellular identity, and disease development. Here, we present a high-throughput cleavage under targets and release using nuclease (CUT&RUN) protocol performed in 96-well plate format. We describe steps for targeted DNA enrichment, high-quality library preparation, sequencing, and data analysis. This protocol enables high-resolution genomic mapping of proteins of interest and factors that define the epigenome. For complete details on the use and execution of this protocol, please refer to Skene and Henikoff 1 and Meers et al. 2

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104631

Authors: Viswakalyan Kotapalli, Polina Iakova, Yue Lu, Abhinav K. Jain

Institution: The University of Texas MD Anderson Cancer Center

Language: English

Topic: Genomics and Chromatin Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funder: University of Texas MD Anderson Cancer Center