Work

Cathepsin G and neutrophil elastase control Leishmania infantum parasitism through TLRs-dependent induction of IFN-α and TNF-α

Year: 2026

Type: article

Abstract: Leishmania infantum and Leishmania donovani are protozoan parasites that cause visceral leishmaniasis, a potentially fatal disease targeting the liver, spleen and bone marrow. To survive, these parasites manipulate host macrophage microbicidal responses and metabolic pathways. Here, we demonstrate that the host serine proteases neutrophil elastase (NE) and cathepsin G (CG), acting in concert with Toll-like receptors TLR2, TLR3, and TLR4, induce the inflammatory cytokines IFN-α and TNF-α to control L. infantum infection in macrophages. Conversely, in vivo experiments revealed that while NE and CG are necessary for inflammatory responses, including IL-12, nitric oxide, and CCL2 production, they unexpectedly promote splenic parasitism. CG-deficient mice failed to recruit Ly6C hi myeloid cells to the spleen, providing a potential mechanism for their reduced parasite burden. Finally, our data suggest that unlike L. donovani , L. infantum cannot hijack cellular host serine peptidases and TLR signalling for survival, yet it remains dependent on these host enzymes to establish systemic spleen infection.

Source: Communications Biology

DOI: 10.1038/s42003-026-10555-8

Authors: Amy Goundry, Aislan C. Vivarini, Nathalia S. Rodrigues, Ulisses G. Lopes, Jeremy C. Mottram, Ana Paula C. A. Lima

Institutions: Universidade Federal do Rio de Janeiro, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal Fluminense, University of York

Language: English

Topic: Research on Leishmaniasis Studies

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $4,190