Protocol for quantitative live-cell imaging of early GPCR trafficking using acid-stable afCFP-Venus FRET
Year: 2026
Type: article
Abstract: Here, we present a protocol to visualize and quantify β-arrestin-dependent signaling of the angiotensin II type 1 receptor (AT1R). We describe steps for detecting receptor-β-arrestin interactions during early trafficking events under our imaging conditions using an afCFP-Venus FRET pair. We then detail procedures for visualizing early intracellular signaling dynamics following AT1R activation and for analyzing GPCR trafficking and signaling dynamics in living cells.
Source: STAR Protocols
Institutions: The University of Osaka, University of Toronto
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $1,550
OpenAlex ID: W7166722276