Translating CRISPR-based Diagnostics to the Clinical Use: Strategies for Practical, Amplification-free Platform
Year: 2026
Type: article
Abstract: Recent advances in molecular diagnostics enable precise and objective identification of pathogens at the molecular level.Among these, the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas system, in particular, is rapidly emerging as a next-generation molecular diagnostic platform, due to its inherent specificity and programmability.However, constraints in guide RNA (guide ribonucleic acid, gRNA) design, including PAM (protospacer adjacent motif) site requirements and target secondary structure considerations, and insufficient sensitivity continue to hinder its clinical implementation.This review provides a comprehensive overview of CRISPR-based diagnostics for both DNA and RNA targets, with a focus on strategies to overcome PAM constraints, improve target accessibility, and reduce the dependence on pre-amplification.Furthermore, we propose prospective avenues for enhancement, including the integration of sample pretreatment, deep learning-guided gRNA design, and the advancement of multiplexing systems.These advances are expected to accelerate the application of CRISPR diagnostic systems from laboratory research tools into practical clinical platforms, facilitating rapid and effective responses to emerging infectious diseases.
Source: KSBB Journal
Authors: Dae Young Shim, Doeon Sung, Jiyoung Park, Jeong Wook Lee
Institution: Pohang University of Science and Technology
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7166727772
FWCI:
Cites:
Cited by:
Related to: