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Protocol for isolating human extracellular vesicles from nephrotic urine and enriching autoimmunoglobulin-triggered extracellular vesicles

Year: 2026

Type: article

Abstract: This protocol describes the isolation of total urinary extracellular vesicles (EVs) from nephrotic patient urines and enrichment of AutoImmunoglobulin-Triggered Extracellular Vesicles (AIT-EVs). Procedures for urine collection, sample processing, buffer preparation, antibody coupling to DynaBeads, urine ultrafiltration, and total EV isolation are provided. The protocol further details EV concentration measurement and selective AIT-EV capture and elution using anti-human IgG4 antibodies. This approach enables reproducible enrichment of AIT-EVs and can be adapted to isolate other EV subtypes by substituting the capture antibody.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104661

Authors: Karen Lahme, Catherine Meyer-Schwesinger

Institution: University Medical Center Hamburg-Eppendorf

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550