Supplementary Figure S3 from Development of a Clinical Assay to Guide Patient Therapy in HPV-Associated Head and Neck Cancer
Year: 2026
Type: supplementary-materials
Abstract: Supplemental Figure 3. Boxplot of NF- κB values across the three cluster subtypes. By unsupervised consensus clustering and self-organizing map (SOM) approaches, we identified three distinct biological subtypes within HPV-positive HNSCC: Cl1 (immune-related), Cl2 (epithelial-mesenchymal transition [EMT]-related), and Cl3 (proliferation-related). This classification holds prognostic significance, with Cl1 exhibiting the most favorable survival outcomes, Cl2 associated with the poorest prognosis, and Cl3 displaying an intermediate survival rate [Locati, 2019]. In contrast to recent studies that classified HPV-related HNSCC into immune and keratinocyte subtypes, our analysis confirmed the immune-associated subtype while further resolving the keratinocyte group into two distinct clusters with differing biological profiles and prognostic implications. NF-κB signature is significantly activated in Cl1-immune related cluster compared to the other 2 clusters: A) Metanalyisis-HPV267 cohort (1); B) BD2-HPV286 cohort (2).
Authors: Sri Vemulamanda, Aditi Kothari, Loris De Cecco, Sulgi Kim, Aditi Kulkarni, Pragati Upadhyay, Stefano Cavalieri, Christophe Courtine, Yasine Mirmozaffari, Jane Brown, Andrew Sewell, Yael Flamand, Yue Xie, Lili Zheng, Shanthi Marur, Trevor Hackman, Bart Singer, John Ayman Hakim, Lori Ramkissoon, Yan Li, Hina Rehmani, Xue Li, Siddharth Sheth, Xuguang Chen, Jennifer Dorth, Quintin Pan, Evan Graboyes, Bhishamjit Chera, Barbara Burtness, Robert L. Ferris, Rene Leemans, Ruud H. Brakenhoff, Frank Hoebers, Kathrin Scheckenbach, Tito Poli, Lisa Licitra, Wendell G. Yarbrough, Natalia Issaeva, Travis P. Schrank
Language: English
Topic: Head and Neck Cancer Studies
Subfield: Otorhinolaryngology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7167067567