Supplemental Figure 4. from Comprehensive Multiplatform Tyrosine Kinase Profiling Reveals Novel Actionable FGFR Aberrations across Sarcomas Affecting the Young
Year: 2026
Type: supplementary-materials
Abstract: A) PDGFA-D ligand RNA expression (TPM) across sarcoma samples, rank ordered on total PDGF-expression counts. RNA expression (Z-score) for B) PTK7, C) FGFR4 and D) FGF8 in the ZERO cohort. Colors indicate specific samples as shown in legends. E-F) Correlation between E) FGFR4 (TPM) and FGFR4 copy number, or F) FGF8 (TPM) and FGF8 copy number in ZERO sarcoma patients. G) Gene set enrichment analysis (GSEA) was performed using the camera method (limma package, R) on the full transcriptomic dataset to compare FP-RMS with other sarcoma subtypes. Pathway definitions were obtained from the Reactome gene set collection (reactome.org). The analysis was conducted using default parameters and significance was assessed based on FDR-adjusted p-values. H) Heatmap illustrating single sample GSEA (ssGSEA) scores for individual sarcoma patient samples from the three FGFR4 pathways identified from (G). Colors indicate specific samples as shown in legends. EWS: Ewing sarcoma, FP-RMS: fusion-positive rhabdomyosarcoma, FN-RMS: fusion-negative rhabdomyosarcoma, OS: osteosarcoma, MRT: malignant rhabdoid tumor, MPNST: malignant peripheral nerve sheath tumor, ASPS: alveolar soft part sarcoma, AS: angiosarcoma, IFS: infantile fibrosarcoma, DSRCT: desmoplastic small round blue cell tumor, GIST: gastrointestinal stromal tumor, SynS: synovial sarcoma, GNET: gastrointestinal neuroectodermal tumor, HGU: high-grade undifferentiated sarcoma, AF: ameloblastic fibrosarcoma, CIC: CIC-rearranged sarcoma, EPI: epithelioid sarcoma, FMX: low-grade fibromyxoid sarcoma, Round cell: EWSR1-PATZ1 round cell sarcoma, desmoid: desmoid tumor.
Authors: Ashleigh M. Fordham, Lauren M. Brown, Chelsea Mayoh, Alice Salib, Zara A. Barger, Marie Wong, Terry C.C. Lim Kam Sian, Changyuan Hu, Jinhan Xie, Kate Gunther, Peter Trebilcock, Rachael L. Terry, Paulette Barahona, Pamela Ajuyah, Alexandra Sherstyuk, Anica Avila, Roxanne Cadiz, Callum M. Perkins, Andrew J. Gifford, Jie Mao, M. Emmy M. Dolman, Andrea Zhao, Luke P. O’Regan, Daniel Gorgels, Loretta M.S. Lau, David S. Ziegler, Michelle Haber, Vanessa Tyrrell, Richard B. Lock, Mark J. Cowley, Wayne Nicholls, Roger J. Daly, Paul G. Ekert, Emmy D.G. Fleuren
Language: English
Subfield: Pulmonary and Respiratory Medicine
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7167092433