Work

Directed evolution of a cytochrome P450 monooxygenase for improved perillyl alcohol biosynthesis via a tailored genetically encoded biosensor

Year: 2026

Type: article

Abstract: A tailored CymR-S77L biosensor enabled high-throughput evolution of a P450 for selective perillyl alcohol production from limonene, establishing a platform for terpene hydroxylation engineering.

Source: RSC Advances

DOI: 10.1039/d6ra03129c

Authors: Catherine A. Odhiambo, Alexandra A. Malico, Gavin J. Williams

Institution: North Carolina State University

Language: English

Topic: Enzyme Catalysis and Immobilization

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,042

Funders: National Institutes of Health, National Institute of General Medical Sciences

Awards: Directed Evolution of Isoprenoid Biosynthesis, NC State Molecular Biotechnology Training Program (MBTP), NC State Chemistry of Life Training Program (CLTP)

OpenAlex ID: W7167252865

FWCI: 2.044

Cites: 64

Cited by: 1

Related to: 10