iPSC neurodegenerative disease initiative isogenic CAG repeat iPSC line for Huntington's disease
Year: 2026
Type: article
Abstract: Purpose of Research: in the KOLF2.1J background as an additional disease series within the iPSC Neurodegenerative Disease Initiative (iNDI) collection. Major Findings: KO, were capable of differentiating to striatal and cortical neurons, astrocytes and microglia using established protocols. General quality control stains and morphological analyses are described for each differentiation. A selected set of assays were carried out on differentiated cells; expanded repeat expressing astrocytes showed altered expression of astrocyte protein markers and morphological characteristics, and striatal neurons showed altered DARPP-32/CTIP2 colocalization. mRNAseq carried out for striatal neurons showed high similarities in gene expression changes between 79Q and KO lines compared to the unexpanded repeat. Conclusions: The KOLF2.1J isogenic CAG repeat series serves as a community resource to study HD mechanisms with the potential for direct comparison across other neurodegenerative diseases through the iNDI collection.
Source: Journal of Huntington s Disease
Language: English
Subfield: Cellular and Molecular Neuroscience
Field: Neuroscience
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,150
Awards: G1095090915, G3523869394, G3549828030, G4953466824, G5158661064, G6037676544, G613752374, Effect of mutant HTT on the development and maturation of astrocytes, G7794782823, G9000007909, G9000077941, G9000200224, Cancer Center Support Grant, Neuroimmunology Training Program at the University of California, Irvine, Training Program in Stem Cell Translational Medicine for Neurological Disorders
OpenAlex ID: W7167372976
FWCI:
Cites:
Cited by:
Related to: