Work

Antibody Binding to Peptide Conjugated Gold Nanoparticles Preferring Dense Antigen Coating with Small Deflection Angle

Year: 2026

Type: article

Abstract: A 13 nm gold nanoparticle (G13) coated with varying densities of His₆ peptide (as a model antigen) was used to study how antigen presentation affects antibody binding, assessed by dynamic light scattering and fluorescence-quenching assays. We found that antigen density and deflection angle strongly influence antibody binding to the G13-His₆ conjugates, yielding over a 120-fold increase in apparent affinity as the His₆ density increases from 2% to 10% (His 6 deflection angle reduces from 37 o to 16 o).

Source: ACS Nanoscience Au

DOI: 10.1021/acsnanoscienceau.6c00049

Authors: Xinyu Ning, Yuan Guo, Dejian Zhou

Institution: University of Leeds

Language: English

Topic: Gold and Silver Nanoparticles Synthesis and Applications

Subfield: Electronic, Optical and Magnetic Materials

Field: Materials Science

Domain: Physical Sciences

Open Access status: gold

Estimated APC paid: $3,500

Funder: Biotechnology and Biological Sciences Research Council