Figure 1 from Tumor-Associated Platelets Suppress T-cell Function and Promote Immune Evasion in TNBC via the P-selectin/P-selectin Glycoprotein Ligand 1 Pathway
Year: 2026
Type: other
Abstract: Tumor-associated platelets (TAP) exhaust CD4+ and CD8+ effector mouse T cells. A, Schematic of the T-cell isolation and platelet coculture experiment. Flow cytometry was used to quantify the functionality of (B) CD4+ and (C) CD8+ effector T cells and exhaustion of (D) CD4+ and (E) CD8+ effector T cells. SF RPMI, serum-free RPMI. F, Schematic of the T-cell and AT-3 coculture experiment. Levels of Zombie NIR caspase 3/7 dye detected over 48 hours. Flow cytometry to detect death in (G) CD3+ T cells and (H) AT-3 tumor cells. Data are representative of three independent experiments. Statistical significance was determined using a one-way ANOVA followed by the Tukey multiple-comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.
Language: English
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7167889253