Work

Protocol for establishing rectal tumor organoid-derived monolayers in a microphysiological system to model radiotherapy

Year: 2026

Type: article

Abstract: Patient-derived organoids offer greater human translational relevance than mouse models or immortalized cell lines, owing to their multicellular composition and patient-specific responses. Here, we present a protocol for establishing a human in vitro rectal tumor model for radiotherapy studies using patient-derived tumor organoids (PDTOs) and monolayer derivatives integrated into a microphysiological system (MPS). We describe steps for cultivating PDTOs, their transition into monolayers, and transfer into an MPS. This protocol enables radiotherapy modelling, toxicity testing, and adjuvant therapies screening.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104708