Work

Protocol for reconstructing 3D human skin equivalents on endogenous dermal matrix with optional pigmentation

Year: 2026

Type: article

Abstract: Previous in vitro 3D skin models are limited by their reliance on animal-derived collagen, a poor representation of the human dermis, and low throughput. Here, we present a protocol for reconstructing 3D human skin equivalents on an endogenous dermal matrix, in 12- and 24-well format, with optional pigmentation. We describe the expansion and seeding of fibroblasts, keratinocytes, and melanocytes in non-contractile polystyrene scaffolds. We then detail air-lifting and epidermal maturation, resulting in a fibroblast-derived dermal matrix with a fully stratified epidermis. For complete details on the execution of this protocol, please refer to Tham et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104718

Authors: Khek-Chian Tham, Zhi Heng Lau, Joycelyn X.Y. Lee, Carine Bonnard

Institutions: Agency for Science, Technology and Research, King Abdullah University of Science and Technology

Language: English

Topic: Skin and Cellular Biology Research

Subfield: Cell Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550