Work

Clinical significance of lncRNA GAS5 in deep vein thrombosis and its mechanism of action on vascular endothelial damage

Year: 2026

Type: article

Abstract: The current research aims to clarify the clinical significance and molecular function of long-non-coding RNA growth arrest-specific 5 (GAS5) in deep vein thrombosis (DVT). 92 DVT patients and 86 controls were included in this study. Real-time quantitative reverse transcription PCR (RT-qPCR) was performed to measure GAS5, microRNA (miR)-134-5p, and forkhead box protein 3 (FOXP3) levels. Receiver operating characteristic (ROC) curves evaluated the diagnostic significance of GAS5 in DVT. Kaplan-Meier and Cox regression analyses assessed the prognostic significance of post-thrombotic syndrome (PTS). An in vitro model using CoCl 2 -induced HUVECs was established to measure cell proliferation, apoptosis, adhesion factors, and inflammatory factors via Cell Counting Kit-8 (CCK-8), flow cytometry, RT-qPCR, and Enzyme-linked immunosorbent assay (ELISA). Protein expression of FOXP3, HIF-1α, and VEGFA was evaluated via Western blot. Dual luciferase report, RIP, and RNA pull-down assays confirmed the direct binding of miR-134-5p to GAS5 or FOXP3. GAS5 and FOXP3 were downregulated in DVT patients’ serum and CoCl 2 -induced HUVECs, while miR-134-5p was upregulated. GAS5 distinguished DVT patients from controls with 89.13% sensitivity and 83.72% specificity. At 18-month follow-up, 32.60% of patients developed PTS, which was more common among those with low GAS5 expression. Mechanistically, GAS5 and FOXP3 bind to miR-134-5p. CoCl 2 enhanced the protein levels of HIF-1α and VEGFA. Higher GAS5 levels reduced CoCl 2 -induced proliferation suppression, apoptosis promotion, and adhesion and inflammatory factors upregulation in HUVECs, but miR-134-5p reversed this effect. GAS5 levels are decreased in DVT patients, serving as a diagnostic and prognostic biomarker. Elevated GAS5 targets miR-134-5p/FOXP3 axis to alleviate endothelial dysfunction and inflammation, thereby reducing thrombosis.

Source: BMC Cardiovascular Disorders

DOI: 10.1186/s12872-026-06197-7

Authors: Hongyu Yang, Shuping Wang, Fei Wang

Institution: Panzhihua University

Language: English

Topic: Cancer-related molecular mechanisms research

Subfield: Cancer Research

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,090