Effects of TiO2 nanoparticles on the kinetics and conformation of bovine alkaline phosphatase
Year: 2026
Type: article
Abstract: This study observed the interaction between titanium dioxide (TiO 2 ) nanoparticles and bovine intestinal alkaline phosphatase (BALP) through a combination of spectroscopic techniques and enzymatic activity assays. Fluorescence spectroscopy suggested that a static quenching mechanism governs the interaction, supported by the binding constant and site number determined at three temperatures. The predominant forces involved in the nanoparticle–protein complex were identified as hydrogen bonding and van der Waals interactions. Circular dichroism analysis indicated substantial alterations in both secondary and tertiary structures of BALP in the presence of TiO 2 . Moreover, enzymatic assays revealed that TiO 2 acts as an uncompetitive inhibitor, reducing the enzyme's activity. These findings provide valuable insight into the conformational and functional consequences of TiO 2 nanoparticle interaction with proteins.
Institutions: Shahrekord University, University of Tehran
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $2,700
OpenAlex ID: W7169653014