Figure 4 from Tissue Context Shapes Distinct Premalignant Outcomes in an HPV16 E6/E7-Mutant Pik3ca Transgenic Mouse Model
Year: 2026
Type: other
Abstract: Morphologic changes developed in the anal and lingual mucosae of K14/PI3K animals. Hematoxylin and eosin (H&E)–stained sections of the anal mucosa (AM) showing epithelial alterations at 2 and 4 months following transgene activation. A, AM from TG-E6/E7 mice displaying stratified squamous epithelium with hyperkeratosis, hypergranulosis, and areas of basal layer stratification (low magnification, 2 months). B, Region of AM exhibiting mild epithelial dysplasia. C, At 4 months, transgenic AM shows squamous epithelium with hyperkeratosis, epithelial hyperplasia, and mild dysplastic changes (low magnification). Inset, higher magnification of the area in C highlighting epithelial hyperplasia and hyperkeratosis. H&E-stained sections of tongue mucosa showing epithelial alterations at 2 and 4 months after transgene activation. D, Low-magnification view of TG-E6/E7 lingual epithelium showing marked hyperkeratosis. Bottom, a higher-magnification image reveals hyperplastic epithelium with cellular and nuclear irregularities in the basal layers and suprabasal hyperkeratosis (2 months). E, Low-magnification view of transgenic tongue epithelium showing hyperkeratosis and mild dysplasia (4 months). F, Area of a K14/PI3K transgenic tongue displaying mild dysplasia. G, Incidence of LSIL and HSIL in the AM of control mice (N = 10) and K14/PI3K animals (N = 7) as the percentage of the total number of animals per group. LSIL, cases diagnosed with mild dysplasia, hyperplasia, and cellular and tissue alterations involving the lower third of the epithelium; HSIL, diagnosed with moderate or severe dysplasia. Incidence of hyperplasia and dysplasia in the tongue epithelium of control mice (N = 10) and TG-E6/E7 animals (N = 9) as the percentage of the total number of animals per group. DISP, dysplasia; HIPER, hyperplasia(); diagnosed with mild, moderate, or severe dysplasia. *, P < 0.0001 by Fisher exact test. H, pS6 expression pattern in the anal canal of K14/PI3K mice (low magnification). Right, a higher-magnification view of the highlighted rectangular area in H shows pS6-positive cells in the suprabasal layers. I, Another area of K14/PI3K AM displaying dysplastic changes and pS6 immunoreactivity. J, Lingual epithelium from a K14/PI3K animal showing pS6 expression in the upper layers. Scale bars, 200 μm (A, C–F, and H); 50 μm (B, I, J, and insets in C, D, and H).
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7170043571
Cites:
Cited by:
Related to: