Work

Rifabutin Cross-resistance and Associated rpoB Mutations in Rifampicin-resistant Mycobacterium tuberculosis Complex Isolates

Year: 2026

Type: article

Abstract: OBJECTIVE: mutations associated with rifabutin susceptibility. MATERIAL AND METHODS: gene were investigated by Sanger sequencing. RESULTS: Among the 43 rifampicin-resistant isolates, 39 (91%) were resistant to rifabutin, while four (9%) remained susceptible. All rifampicin-susceptible isolates were also susceptible to rifabutin. A total of 44 non-synonymous single nucleotide polymorphisms were detected in rifampicin-resistant isolates, whereas no mutations were observed in the control group. Seven distinct mutation patterns were identified, including one isolate with a double mutation. The most common mutations were S531L (76%), H526Y (12%), and D516Y (4%). The mutations D516Y, L511P, and L533P, as well as the M515I+D516Y double mutation, were associated with rifabutin susceptibility. CONCLUSION: studies, including minimum inhibitory concentration testing, and additional clinical data on rifabutin-containing regimens are needed.

Source: Thoracic research and practice

DOI: 10.4274/thoracrespract.2026.2026-1-16

Authors: Nalan Sürücüoğlu, Süheyla Sürücüoğlu, Nuri Özkütük

Institution: Manisa Celal Bayar University

Language: English

Topic: Tuberculosis Research and Epidemiology

Subfield: Infectious Diseases

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: diamond

OpenAlex ID: W7170067254