Work

Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles

Year: 2026

Type: article

Abstract: Extracellular vesicles (EVs) are gaining increasing attention among studies focusing on biomarker research, elucidating disease mechanisms, and exploring therapeutic approaches. Here, we present a method for size-exclusion chromatography (SEC)-based enrichment of human plasma EVs, and describe steps for assessing the quality of obtained EV samples. For characterizing the EVs, we provide detailed procedures for western blot, transmission electron microscopy, and nanoparticle tracking analysis techniques.

Source: STAR Protocols

Authors: Fanni Annamária Boros, Philipp Arnold, Martin Regensburger, Friederike Zunke

Institutions: Friedrich-Alexander-Universität Erlangen-Nürnberg, Universitätsklinikum Erlangen

Language: English

Topic: Extracellular vesicles in disease

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550

Funders: Michael J. Fox Foundation for Parkinson's Research, Deutsche Forschungsgemeinschaft