Work

Single-nucleus RNA sequencing of miBrains, a human brain tissue model

Year: 2026

Type: dataset

Abstract: This dataset contains snRNA-seq data generated from human induced pluripotent stem cell-derived 3D brain tissue. Specifically, this is a multicellular integrated human brain tissue (miBrain) containing multiple brain cell types. The dataset includes three experimental conditions: control miBrains, miBrains with overexpression and mutation of the SNCA gene (A53T) and the APOE3/3 genotype, and miBrains with SNCA-A53T and APOE4/4 genotype. The SNCA manipulation is present only in the neurons, whereas the APOE genotype manipulation is identical in all cell types within the same sample. Control miBrains express endogenous SNCA and harbor APOE3/3. All miBrains are isogenic.

Source: Zenodo (CERN European Organization for Nuclear Research)

DOI: 10.5281/zenodo.21510592

Authors: Mesentier-Louro, Louise A., Goldman, Camille, Buonfiglioli, Alice, Kyriakis, Dimitrios, Fullard, John F., Hennigan, Evelyn, Lee, Donghoon, Roussos, Panos, Blanchard, Joel W.

Institution: Icahn School of Medicine at Mount Sinai

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

OpenAlex ID: W7170145959

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