Year: 2026
Type: preprint
Abstract: Alternative splicing is an important regulatory layer in gene expression, but knowledge on the isoform protein molecules continue to lag their canonical counterparts. An open question is whether alternative protein isoforms feature different half-life than the canonical counterpart, which could indicate differential usage and functional diversification. Here we combined a proteogenomics approach with heavy water-based protein turnover analysis to survey 24 pairs of canonical-alternative protein isoforms in the mouse heart. The results provide a reference on their numerical half-life and also reveal widespread differences in isoform stability.
Language: English
Subfield: Spectroscopy
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
Awards: G1086261101, G5424111711, CC* Compute: HPC Services for the Colorado State University System, G7852733817, CC* Regional Computing: Enhancing Computing At Regional Schools in the Rocky Mountain Advanced Computing Consortium (RMACC), G8054170119, G8067353062, Alternative Protein Isoforms in Ventricular Remodeling, Post-transcriptional regulations of proteomes in stress and senescence, Investigations of proteome turnover kinetics under cellular differentiation
OpenAlex ID: W7170284908
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