Work

Protocol to induce memory generalization and stress-like behaviors without memory strengthening using contextual fear conditioning in mice

Year: 2026

Type: article

Abstract: Over-generalization of negative memory is common in psychiatric disorders, including depression and anxiety. Here, we present a protocol to dissociate memory generalization from memory strengthening and induce lasting stress-related behavioral alterations using contextual fear conditioning in adult mice. We describe the steps for repeated conditioning in identical or distinct contexts, followed by memory testing and behavioral assays of stress-related phenotypes. This protocol enables the investigation of the behavioral and biological mechanisms underlying pathological memory generalization and its consequences. For complete details on the use and execution of this protocol, please refer to Cheng et al. 1

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104741

Authors: Xin Cheng, Yan Zhao, Yubo Hu, Wei-Jye Lin, Xiaojing Ye

Institutions: Sun Yat-sen University, Sun Yat-sen Memorial Hospital

Language: English

Topic: Memory and Neural Mechanisms

Subfield: Cognitive Neuroscience

Field: Neuroscience

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $1,550