Work

Preliminary Single-Center Evaluation of the Alifax Molecular Mouse Sepsis Panel for Rapid Detection of Bloodstream Pathogens

Year: 2026

Type: article

Abstract: Background/Objectives: We evaluated the diagnostic performance of the Alifax Molecular Mouse (MM) sepsis panel for rapidly directly detecting pathogens and resistance genes in positive blood culture broths. Methods: In this prospective single-center study, 40 positive blood culture specimens were analyzed using four MM cartridges targeting Gram-positive and -negative bacteria and major antimicrobial resistance genes. The results were compared with those obtained using the routine microbiological protocol, including organism identification using the MALDI Biotyper system and antimicrobial susceptibility testing using the VITEK 2 and MicroScan WalkAway systems. Additionally, the MM panel results were compared with those obtained using the FilmArray BCID2. Results: Compared with the routine protocol, the MM panel achieved a positive percent agreement (PPA) of 89.5% (34/38; 95% confidence interval [CI], 75.9–95.8%) for bacterial identification, indicating moderate-to-good diagnostic performance. For antimicrobial resistance gene detection, the PPA was 92.1% (35/38; 95% CI, 79.2–97.3%). Compared with the FilmArray BCID2, the overall agreement of the MM panel was 89.4% (34/38) for bacterial identification and 84.2% (32/38) for antimicrobial resistance gene detection. The MM panel generated clinically actionable results within 1 h (mean turnaround time, 55.5 min; 95% CI, 55.1–55.9 min), substantially reducing the turnaround time compared with the conventional microbiological workflow, which requires 1.5–2 days for subculture and automated antimicrobial susceptibility testing. Conclusions: The MM sepsis panel enabled rapid, direct identification of bloodstream pathogens and key antimicrobial resistance markers from positive blood cultures, substantially reducing turnaround time and exhibiting its potential as an adjunct to routine microbiological workflows.

Source: Diagnostics

DOI: 10.3390/diagnostics16152369

Authors: Jay Ho Han, Jae Kwon Kim, Jung Ok Kim, Jung Suk Lee, Sungjin Jo

Institutions: The Catholic University of Korea Uijeongbu St. Mary's Hospital, Catholic University of Korea, The Catholic University of Korea Seoul St. Mary's Hospital, The Catholic University of Korea Eunpyeong St. Mary's Hospital

Language: English

Topic: Bacterial Identification and Susceptibility Testing

Subfield: Clinical Biochemistry

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,137