Elevated TGF-β/IL-10 in platelet-monocyte complexes exacerbates atherosclerosis in people with HIV 2252272
Year: 2026
Type: article
Abstract: Introduction People living with HIV (PLWH) are more susceptible to atherosclerosis (AS). Here, we examine the functional consequences of platelet-monocyte complexes (PMCs), which are elevated in PLWH and are hypothesized to contribute to HIV-associated comorbidities. Methods We performed single-cell RNA sequencing (scRNAseq) of monocytes isolated from age-, sex-, and cardiovascular risk score-matched participants categorized based on HIV and early AS status (n = 8 per group). Monocytes and platelets were cocultured ex vivo ± LPS (to simulate chronic inflammation) and analyzed at multiple time points as PMCs (24 hours) and PMC-derived macrophages (Mϕs, six days) via bulk RNA sequencing/RT-qPCR, ELISA, and flow cytometry. PMCs were cocultured with primary human endothelial cells (ECs) between three and six days of macrophage differentiation to assess endothelial to mesenchymal transition (EndMT) via RT-qPCR and ELISA. Results scRNAseq PMCs in PLWH and AS had increased TGF-β/IL-10 signaling compared to either PLWH or people living with AS (PLWAS) alone. Ex-vivo PMCs upregulated expression of genes involved in TGF-β signaling and IL-10 expression at both the monocyte and Mϕ developmental states. LPS stimulation increased IL-10 and TGF-β secretion and reduced TNF-α levels in culture supernatants. PMC-derived Mϕs increased expression of costimulatory molecules CD86 and CD80. When cultured with primary ECs, PMC-derived Mϕs reduced Pecam1 and Cdh5 and increased S100a4 expression. CCL2 and IL-6 were elevated in PMC-derived Mϕ-EC cocultures. LPS stimulation of cocultures increased expression of adhesion molecules Icam1 and Vcam. Conclusion Platelet interactions underscore monocyte-Mα¦ differentiation that promotes EndMT via TGF-β and IL-10, suggesting a pro-AS role of these factors in early disease. Enhanced TGF-β/IL-10 expression in PMCs of PLWH and AS suggests an increased relevance of this pathology in these individuals and defines a mechanistic link between aberrant platelet activation, PMC formation, and AS in PLWH. Funding Source T32AI118689, R01HL160229 Topic Categories Innate Immune Responses and Host Defense: Cellular Mechanisms (INC)
Source: The Journal of Immunology
Institution: University of Rochester Medicine
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7171543742
FWCI:
Cites:
Cited by:
Related to: