Variability of adhesin presentation in the cell walls of Candida glabrata and Candida parapsilosis
Year: 2017
Type: conference-paper
Abstract: Non-Candida albicans Candida species have gained significant importance over the past decades becoming one of the principal causes of nosocomial and bloodstream-invasive Candidiasis. The increased prevalence of drug resistance and high capacity to form biofilms on abiotic surfaces makes Candida parapsilosis and Candida glabrata important targets for the development of specific diagnostic or treatment strategies. Both genomes encode a number of potential adhesins whose roles remain uncharacterized. We hypothesized that these might be modulated in clinical isolates conferring different adhesion properties and biofilm formation intensities. We therefore analyzed biofilm formation capacity, presence of putative-novel adhesins and azole susceptibility in clinical isolates of both species. Cell-wall proteomic analyses of two hyperadhesive isolates C. glabrata demonstrated the incorporation of previously undescribed adhesion molecules in addition to a core cell wall proteome. Routine screening of adhesion showed that approx. 20% of clinical C. glabrata and 24% of C. parapsilosis isolates presented a hyperbiofilm-forming phenotype, but no correlation with body site of isolation became evident. An increased number of different adhesins were detected in the cell walls
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7198437638