Resistentit sairaalaperäiset bakteerit beniniläisen sairaalan äitiys- ja neonataaliosastoilla ja potilaissa
Year: 2025
Type: article
Study design: Observational Study
Abstract: Antimicrobial resistance (AMR) poses a serious threat to maternal and neonatal health in low-resource settings, where infection surveillance and prevention remain limited. This study investigated resistance gene profiles and potential transmission routes in the maternity and neonatology wards of Menontin Hospital, Benin, providing baseline data for the EU-funded Mimin project. Samples were collected from mothers, newborns, healthcare workers, and high-touch hospital surfaces. Bacterial isolates were obtained using CHROMagar™ ESBL and mSuperCARBA™ selective media, followed by whole-genome sequencing, resistance gene screening, and assessment of genomic relatedness. Genomic similarity between isolates was analyzed to trace possible transmission pathways. Among 77 isolates, the most clinically relevant isolates in this study were Klebsiella pneumoniae, Escherichia coli, Acinetobacter baumannii, and Pseudomonas lactis. Extended-spectrum β-lactamase (ESBL) genes were widespread among Enterobacteriaceae, with blaCTX-M-15 detected in many isolates. In A. baumannii, resistance was determined by OXA-type carbapenemase genes (blaOXA-51, blaOXA-106, blaOXA-120) and the cephalosporinase gene blaADC-25. Carbapenemase genes blaNDM-1 and blaNDM-7 were detected in Acinetobacter rongchengensis, Enterobacter cloacae, and Atlantibacter species. Sourmash analysis revealed clusters of highly similar isolates shared across mothers, newborns, healthcare workers, and environmental surfaces, suggesting transmission within the hospital environment and persistence of multidrug-resistant strains in both human and environmental reservoirs. The coexistence of multiple resistance determinants, combined with evidence of hospital-associated transmission, highlights the urgent need for strengthened infection prevention, improved cleaning practices and antimicrobial stewardship in Beninese maternity wards.
Source: Työväentutkimus Vuosikirja
Author: Uusihakala, Serafiina
Language: English
Subfield: Microbiology (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7199280064