Figure 1 from Glioblastoma Subtypes Exhibit Distinct Migration Mechanics and Immune Responses
Year: 2026
Type: other
Abstract: De novo induced GBM mouse models using immunocompetent mice recapitulate mesenchymal and proneural subtypes of human GBM. A, Schematic of mouse models. Plasmids encoding oncogenic drivers NRASG12V or PDGFβ in combination with SV40LgTA were injected into P1 FVB mice to induce mesenchymal and proneural high-grade gliomas, respectively. B, Hematoxylin and eosin (H&E) and Ki67 IHC staining of NRAS and PDGF tumor sections. Scale bars, 50 μm. C, Unsupervised hierarchical clustering of mRNA expression in induced mouse tumors and human IDH-WT GBM-TCGA. Arrows indicate conserved genes present in both mouse and human gene clusters as defined by systematic comparison of gene cluster membership between datasets. D, Quantification of relative expression of mouse gene clusters within the mouse dataset (top) and human GBM molecular subtypes (bottom). E and F, Relative expression of key mesenchymal and proneural genes within mouse tumors (E) and human tumors (F). Solid and dashed lines represent mean and median values, respectively. Error bar represents the SEM. +, P < 0.05; *, P < 0.01; **, P < 0.001; ***, P < 0.0001; ****, P < 0.00001, Statistical significance was assessed using one-way ANOVA. [A, Created in BioRender. Li, R. (2026) https://BioRender.com/49xmcik.]
Authors: Ghaidan A. Shamsan, Chao J. Liu, Brooke C. Braman, Ruyi Li, Susan K. Rathe, Aaron L. Sarver, Nima Ghaderi, Mariah McMahon, Rebecca L. Klank, Barbara R. Tschida, S. Joseph McFarren, Pamela C. Rosato, David Masopust, Jann N. Sarkaria, H. Brent Clark, Steven Rosenfeld, David A. Largaespada, David J. Odde
Subfield: Genetics
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7202207565
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