Work

miR-511 suppresses non-small cell lung cancer by targeting EREG

Year: 2026

Type: article

Abstract: BACKGROUND: miR-511 is a well-known tumor suppressor in many tumor types. However, whether miR-511 directly regulates EREG in non-small cell lung cancer (NSCLC) and the functional consequences of this regulation have not been investigated. METHODS: Real-time quantitative polymerase chain reaction (qRT-PCR) was used to measure miR-511 expression. The proliferation, invasion and migration abilities of NSCLC cell lines A549 and H460 were determined by CCK-8, colony formation, and Transwell assays. RNA-seq was used to screen the target genes of miR-511. A xenograft mouse model was established to validate the tumor-suppressive role of miR-511 in vivo. RESULTS: The expression of miR-511 is significantly down-regulated in NSCLC tissues and cells. The inducing of miR-511 dramatically inhibit the proliferation, invasion and migration of NSCLC cells. TargetScan predicates that EREG might be a target gene of miR-511. Dual-luciferase reporter assay confirmed that miR-511 directly binds to the 3'-UTR of EREG. Induction of MiR-511 significantly down-regulated the mRNA and protein levels of EREG, thus validating the prediction. Moreover, rescue experiments show that EREG overexpression impairs the effect of miR-511 on suppressing the malignant phenotype of NSCLC cells. Furthermore, in vivo xenograft experiments demonstrated that miR-511 overexpression significantly suppressed NSCLC tumor growth and reduced EREG expression in tumor tissues. CONCLUSION: miR-511 inhibits the proliferation and metastasis of NSCLC by down-regulating the expression of EREG. The miR-511-EREG axis may provide a potential basis for future therapeutic exploration in NSCLC.

Source: Journal of Cardiothoracic Surgery

DOI: 10.1186/s13019-026-04508-9