Work

Intact Cell Dissociation of Formalin-Fixed Paraffin-Embedded Colon Tissue Enhances Cell Recovery and Gene Detection in Single-Cell RNA Sequencing

Year: 2026

Type: article

Abstract: [Figure: see text]

Source: Cellular and Molecular Gastroenterology and Hepatology

DOI: 10.1016/j.jcmgh.2026.101863

Authors: James R. Evans, Alan J. Simmons, Zhengyi Chen, Monica E. Brown, Matthew E. Bechard, Morad C. Malek, Cody N. Heiser, Frank Revetta, M. Kay Washington, Laura P. Hale, Robert J. Coffey, Ken S. Lau

Institutions: Vanderbilt University, Vanderbilt University Medical Center, Duke University, Duke Medical Center, Vanderbilt-Ingram Cancer Center

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $3,430

Funders: National Science Foundation, Vanderbilt University, Vanderbilt-Ingram Cancer Center, Vanderbilt University Medical Center, National Institutes of Health, National Cancer Institute, National Institute of Allergy and Infectious Diseases, National Institute of Diabetes and Digestive and Kidney Diseases

Awards: Mechanisms controlling distinct growth and functional characteristics of the perinatal and adult thymus, Graduate Research Fellowship Program (GRFP), G1937063530, Tuft cell heterogeneity in function, lineage, and structure in ileal inflammatory disease, G3502523572, G4195101910, G9000046513, G9000117310, G9000150437, A Multimodal 3D Atlas of Colorectal Cancer Across Ages of Onset, Shaping of the Microenvironment in Colonic Pre-Cancer by Epithelia and Microbiota, Vanderbilt-Ingram Cancer Center SPORE in Gastrointestinal Cancer

OpenAlex ID: W7202381759

FWCI:

Cites: 10

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