Supplementary Figure S2 from Reprogramming of TLR–Ferroptosis Signaling and Immunometabolic Pathways Overcomes Myeloid Suppression to Improve Checkpoint Blockade in Prostate Cancer
Year: 2026
Type: supplementary-materials
Abstract: PSMAi-C' dots trigger proinflammatory responses in prostate cancer and immune cells. (a) Gene expression levels, as fold changes over controls, using qRT-PCR and (b) normalized cytokine and chemokine expression levels by Proteome Profiler in Myc-CaP cells and supernatants, respectively, following incubation with 15 uM of PSMAi-C’ dots over 72 h. (c) IFN-a/b reporter B16 cell luminescence (OD620) after incubation with supernatants from particle-treated and untreated Myc-CaP cells. Percentage (%) of surface-expressed (d) IFNGR1, (e) MHC-I, (f) PD-L1, and (g) CD73 on vehicle- and particle-treated Myc-CaP cells by flow cytometry at 72-hour post-exposure. (h) Flow cytometry-based analyses of the %M1 and %M2 markers in vehicle- and particle-treated bone marrow-derived macrophages (BMDMs) over 72 h. (i) IFN-a/b reporter B16 cell luminescence (OD620) after incubation of BMDMs with supernatants from (h). Percentage of CD8+ T cell-specific (j) IFN-g and (k) TNF-a secretion from splenocytes by flow cytometry following a 72-hour incubation with or without PSMAi-C’ dots. (L) T cell cytotoxicity (expressed as luciferase (luc+) activity) following co-culture of mouse T cells with luc + Myc-CaP cells, with and without PSMAi-C’ dots for 48 h. All samples were run in triplicate. Numerical data are presented as mean ± s.e.m. 1-way ANOVA with Kruskal-Wallis test was performed in (a). Unpaired t-tests were performed in (d–g) and (i–l). 2-way ANOVA with Šídák's multiple comparisons test was performed in (c) and (h). *p < 0.05, **p < 0.01, ****p < 0.001.
Authors: Nabil A. Siddiqui, Li Zhang, Gabriel DeLeon, Nada Naguib, Tuo Zhang, Thomas P. Quinn, Feng Chen, Virginia Aragon Sanabria, Anusha Aditya, Daniel Penarete, Sebastien Monette, Fabio Socciarelli, Miles Pourbaghi, Rachel Lee, Chan Lee, Pat B. Zanzonico, Howard I. Scher, Michael Overholtzer, Ulrich Wiesner, Jedd D. Wolchok, Michelle S. Bradbury
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7203445623
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