Supplementary Figure S1 from Reprogramming of TLR–Ferroptosis Signaling and Immunometabolic Pathways Overcomes Myeloid Suppression to Improve Checkpoint Blockade in Prostate Cancer
Year: 2026
Type: supplementary-materials
Abstract: Tunable structural properties of ultrasmall PSMAi-PEG2-Cy5-C’ dots promote enhanced binding/uptake and potency in prostate cancer cell lines. (a) C’ dot-encapsulating deep red fluorescent dye Cy5 (1), surface functionalized with DFO chelator (2) and the PSMA targeting peptide, PSMAi (3). (b) Chemical structure of PSMAi-PEG2 peptide. (c) Cell binding of PSMAi-PEG-C’ dots and second generation PSMAi-PEG2-C’ dots in LNCaP cells. (d) FCS correlation curve and fit of PSMAi-PEG2-Cy5-C’ dots. (e) GPC elugram of PSMAi-PEG2-Cy5-C’ dots with Gaussian fit. (f) Competitive binding curves for various PSMAi-targeted C’ dot constructs versus PSMAi peptide in LNCaP cells. (g) Flow cytometry of concentration-dependent PSMAi-PEG2-Cy5-C’ dots particle uptake (4 h) in human and murine prostate cancer cell lines, expressed as mean fluorescence intensity (MFI). (h) Specific uptake of PSMAi-PEG2-Cy5-C’dots in LNCaP, Myc-CaP, and PC-3 cells without and with addition of anti-PSMA antibody by flow cytometry, expressed as MFI. (i) Differential PSMA expression in LNCaP, Myc-CaP, PC-3, and ID8 cells by western blot. (j) Confocal microscopy of PSMAi-PEG2-Cy5-C' dot uptake in LNCaP cells (Red: particle, Green: 70 kDa FITC-dextran, Blue: DAPI). All numerical data are presented as mean ± s.e.m. (n = 3); scale bar: 5 um. Unpaired t-tests were performed in (c) and (h). **p < 0.01, ***p < 0.005, ****p < 0.001, ns – not significant.
Authors: Nabil A. Siddiqui, Li Zhang, Gabriel DeLeon, Nada Naguib, Tuo Zhang, Thomas P. Quinn, Feng Chen, Virginia Aragon Sanabria, Anusha Aditya, Daniel Penarete, Sebastien Monette, Fabio Socciarelli, Miles Pourbaghi, Rachel Lee, Chan Lee, Pat B. Zanzonico, Howard I. Scher, Michael Overholtzer, Ulrich Wiesner, Jedd D. Wolchok, Michelle S. Bradbury
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7203449962
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