Additional file 1 of NSCLC brain metastases exhibit reduced HLA-I antigen presentation machinery and immune evasion independent of IFNγ signaling defects
Year: 2026
Type: article
Abstract: Supplementary Material 1: Supplementary Figure S1. B2M protein levels across normal tisues. A) QIF scores of B2M in the total tissue are of a lymph node, tonsil, kidney, placenta, and brain tissue, respectively. The graphs depict the mean +- SEM of all scores in each group. B) Representative immunofluorescence image illustrating B2M high protein expression in a lymph node (A) and B2M negative expression in a normal brain tissue (right). Supplementary Figure S2. A) Levels of CD8+ (left), CD4+ (center), and FOXP3+ TILs (right panel) in samples of LBM from Cohort 1 classified by cancer cell β2M unaltered (unalt) versus downregulated (down). B-C) QIF scores of CD8+ (left), CD4+ (center), and FOXP3+ TILs (right panel) measured selectively within the cancer cell compartment (top) and stromal cell compartment (bottom) in samples classified as High (H) vs Low (L) (using the media as the cut point) for B2M (B), pSTAT1 (C) and IRF1 (D) QIF in PLT (black dots) and LBM (white dots) from Cohort 1. The graphs depict the mean ± SEM of all scores in each group. Supplementary Figure S3. Heatmap showing Pearson’s correlation coefficients between the expression of genes upregulated in control (A) and IFNγ treated H2030-BrM3 cells (B) and HLA class-I APM genes. Supplementary Figure S4. Signaling pathways upregulated (green bars) and downregulated (blue bars) in the H2030-BrM3 cell line compared to the parental H2030 cell line in control/unstimulated condition (A) and after INFγ treatment (B).
Source: Figshare
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7203455201
FWCI:
Cites:
Cited by:
Related to: