Supplementary Figure S10 from Reprogramming of TLR–Ferroptosis Signaling and Immunometabolic Pathways Overcomes Myeloid Suppression to Improve Checkpoint Blockade in Prostate Cancer
Year: 2026
Type: supplementary-materials
Abstract: Particle-based therapy reprograms myeloid phenotypes, promotes phagocytosis, and boosts T cell–mediated immune activation in Hi-Myc splenocytes. (a) Schematic depicting the timeline of Hi-Myc tumor development in positively genotyped mice, along with the experimental procedure for isolating/culturing bone marrow-derived macrophages (BMDMs) and splenocytes (T cells) for ex vivo analyses following treatment of mice with saline, PSMAi-C’ dots, ICB, or Dual Tx. (b) Percentage (%) of tumor cells phagocytosed by co-cultured BMDMs using flow cytometry 24 h after exposing the former to treatments specified in (a). Percentage of cells co-expressing F4/80 and CFSE were gated to identify phagocytic macrophages. (c) Fold changes of representative genes in BMDMs exposed to treatments in (a) for 48 h and assayed via RT-qPCR. (d – g) ELISpot assay of IFN-γ, TNF-α, and IFN-γTNF-α secretion from isolated splenocytes (a). Data in (b, c, e – g) are presented as mean ± s.e.m. and a 1-way ANOVA with Brown-Forsythe test was performed. *p < 0.05, **p < 0.01, ****p < 0.001.
Authors: Nabil A. Siddiqui, Li Zhang, Gabriel DeLeon, Nada Naguib, Tuo Zhang, Thomas P. Quinn, Feng Chen, Virginia Aragon Sanabria, Anusha Aditya, Daniel Penarete, Sebastien Monette, Fabio Socciarelli, Miles Pourbaghi, Rachel Lee, Chan Lee, Pat B. Zanzonico, Howard I. Scher, Michael Overholtzer, Ulrich Wiesner, Jedd D. Wolchok, Michelle S. Bradbury
Subfield: Oncology
Field: Medicine
Domain: Health Sciences
Open Access status: gold
OpenAlex ID: W7203468563
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