A polyphenol-rich plant extract reduces oxidative stress in the gastrocnemius muscle of mice with high-fat high-sucrose diet-induced obesity
Year: 2026
Type: article
Abstract: Graphical Abstract Abstract Age-related diseases linked to obesity are often associated with significant oxidative stress. In this context, the antioxidant properties of a polyphenol-rich plant extract (PRPE) were evaluated using complementary biochemical assays and in vivo in the skeletal gastrocnemius muscle of mice treated with PRPE and fed with different diets, i.e. standard (Std) diet or high-fat/high-sucrose (HFHS) diet. We report that PRPE is a potent antioxidant mixture, as shown by its reactive oxygen species (ROS)-scavenging properties using different assays: biological-based KRL (Kit Radicaux Libres, i.e., free radicals kit) and chemical-based assays: DPPH, FRAP, and TEAC. We observed an antioxidant effect in the gastrocnemius muscle from mice fed a high-fat/high-sucrose (HFHS) diet and treated with PRPE: increased antioxidant activities revealed with the KRL assay (expressed in Trolox equivalent), and stimulated expression of genes involved in oxidative stress defense under ROS exposure by RT-qPCR: nuclear respiratory factor 1 (Nrf1), sirtuin 1 (Sirt1), superoxide dismutase 1 (Sod1), and uncoupling protein 2 (Ucp2). These in vivo data, which were lacking until now, are in agreement with earlier results of the in vitro approach reporting significant protective activities of PRPE on the C2C12 skeletal muscle cell line. They confirm the efficacy of the PRPE in the diet of treated animals, especially in skeletal muscle.
Source: Redox Experimental Medicine
DOI: 10.1530/rem-26-0015
Institutions: Laboratoire Bio-peroxIL, Université Bourgogne Europe, Inserm, Université de Bourgogne, Centre de recherche Translationnelle en Médecine moléculaire, Centre National de la Recherche Scientifique, Laboratoire Interdisciplinaire Carnot de Bourgogne, Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement, Centre des Sciences du Goût et de l'Alimentation
Language: English
Subfield: Biochemistry
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7203622641