Work

Effect of shortening Ω-loop D on 3D domain swapping in cytochrome c

Year: 2026

Type: article

Abstract: Three-dimensional domain swapping (3D-DS) properties of wild-type human cyt c and its variants, in which 1 to 5 residues were deleted from Ω-loop D, were investigated. The cyt c dimer stability generally decreased as more amino acids were deleted from Ω-loop D. However, the del82-85 dimer exhibited a relatively small dissociation rate constant and hydrodynamic size, indicating that protein–protein interactions between the functional globular units play a crucial role in determining the stability of the 3D-DS dimer.

Source: Chemistry Letters

DOI: 10.1093/chemle/upag163

Authors: Kotori Ota, Myriam Mahoudi, Tsuyoshi Mashima, Shun Hirota

Institution: Nara Institute of Science and Technology

Language: English

Topic: Protein Structure and Dynamics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $1,003

Funder: Japan Society for the Promotion of Science