Monitoring Tomato Brown Rugose Fruit Virus: Insights from the Nextstrain Build and Candidate Resistance-Breaking Mutations
Year: 2026
Type: article
Abstract: The first detection of tomato brown rugose fruit virus (ToBRFV) in tomato crops in the Netherlands was in 2019. Since then, the National Plant Protection Organization of the Netherlands has conducted surveys to trace and monitor this virus as part of the EU emergency measures up to the end of 2024. An interactive and publicly available ToBRFV Nextstrain build was developed to enable international data sharing and to support outbreak investigations. This report announces the addition of 83 (near) complete ToBRFV genome sequences in the fifth version of the ToBRFV Nextstrain build (available at https://nextstrain.nrcnvwa.nl/ToBRFV ). This announcement highlights the new features of version 5, namely support values for the phylogenetic clustering and display options at the municipality level and compatibility with Nextclade. Furthermore, it demonstrates how genomic data shared within the (international) community supports the reconstruction of introduction pathways, the monitoring of local spread, and the detection of candidate resistance-breaking genotypes. [Formula: see text] Copyright © 2026 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .
Source: PhytoFrontiers™
Authors: Carla Oplaat, Pier P. M. de Koning, Aimee R. Fowkes, Despoina Beris, Gerrit A. Hiddink, Sergio de la Fuente van Bentem, Annelies Haegeman, Kris De Jonghe, Zafeiro Zisi, Sam McGreig, Anna Skelton, Ian P. Adams, Bas Berbers, Christel de Krom, Jerom van Gemert, Naomi te Braak, Lucas van der Gouw, Marcel Westenberg, Bart T. L. H. van de Vossenberg, Christina Varveri, Johanna W. Roenhorst, Martijn Schenk, Adrian Fox, Marleen Botermans
Language: English
Topic: Plant Virus Research Studies
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W7203686905