Work

Protocol for flash-freezing, pooling, and preparing single nuclei suspensions from amniote inner-ear sensory epithelia

Year: 2026

Type: article

Abstract: The inner-ear sensory epithelia are characteristically difficult to dissociate into healthy single-cell suspensions, with adult tissue presenting the most challenges. Recent advances in RNA sequencing have allowed for transcriptomic profiling of single nuclei, instead of single cells, offering a valuable alternative for processing sensitive tissue for single-cell genomics. Here, we provide a detailed protocol for flash-freezing, pooling, and preparing single nuclei suspensions from mouse and chicken inner-ear sensory epithelia, with a focus on optimization and quality control.

Source: STAR Protocols

DOI: 10.1016/j.xpro.2026.104788

Authors: Jimena Perez Lloret, Valeria Carolina Castagna, Marcela Lipovsek

Institution: University College London

Language: English

Topic: Single-cell and spatial transcriptomics

Subfield: Molecular Biology

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: gold

Estimated APC paid: $1,550

Funders: Wellcome Trust, Royal Society, Royal Veterinary College