Biasing Conformational Sampling in AlphaFold 3 and Boltz-2 via Pair Representation Scaling
Year: 2026
Type: article
Abstract: Deep learning has transformed protein structure prediction, yet most systems return a single dominant conformation with little control over the alternative functional states. We introduce pair representation scaling, an inference-time method that biases conformational sampling in diffusion-based structure predictors by multiplying the latent pair representation by a single scalar before the Pairformer trunk, without retraining, an auxiliary model, or a second forward pass. On 86 two-state targets spanning domain motions and membrane transporters, scaling broadens the conformational ensembles of both AlphaFold 3 and Boltz-2 and recovers alternative states that default inference misses, most strongly in AlphaFold 3, where the gains extend even to targets deposited after the training cutoff. It approaches the alternative-state recovery of alignment-based sampling methods, and the benefit persists even without a multiple-sequence alignment. The predicted distance distributions show that scaling shifts the encoded two-state distribution toward the experimentally observed alternative state, a directed modulation rather than an arbitrary perturbation. Pair representation scaling is an interpretable, low-cost handle for the conformational ensembles of deep-learning structure predictors.
Authors: Shosuke Suzuki, Toshiyuki Amagasa
Institution: University of Tsukuba
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: hybrid
Estimated APC paid: $4,500
Awards: G1992475168, G5457231128
OpenAlex ID: W7203764423