Work

Technical note: Micromanipulation flicking technique for embryo biopsy in bovine embryos

Year: 2026

Type: article

Abstract: Embryo biopsy is widely used in human assisted reproduction for preimplantation genetic testing, but its application in bovine in vitro fertilization (IVF) programs remains limited, largely due to the cost and complexity of laser-based systems. Here we describe a micromanipulation method for laser-free embryo biopsy in bovine embryos that relies on a mechanical separation technique to collect embryo samples while preserving embryo viability. This method provides a practical alternative for embryo biopsy in bovine IVF systems and may facilitate the incorporation of embryo genotyping into genetic improvement programs.

Source: Reproductive Biology

DOI: 10.1016/j.repbio.2026.101279

Authors: Clara Slade Oliveira, Naiara Zoccal Saraiva, Dayana Silva Araújo, Luiz Sergio de Almeida Camargo

Institutions: Embrapa Gado de Leite, Universidade Federal de Minas Gerais

Language: English

Topic: Animal Genetics and Reproduction

Subfield: Genetics

Field: Biochemistry, Genetics and Molecular Biology

Domain: Life Sciences

Open Access status: hybrid

Estimated APC paid: $2,750

Funder: Fundação de Amparo à Pesquisa do Estado de Minas Gerais