Work

Mycoplasma mycoides subsp. capri encodes multiple pathways for hydrogen peroxide production

Year: 2026

Type: article

Abstract: ‬‬Summary Several Mollicutes species import glycerol and related molecules as carbon sources, resulting in the release of hydrogen peroxide (H 2 O 2 ), which has been suggested as a pathogenicity mechanism. We systematically deleted genes encoding components of the H 2 O 2 pathways in Mycoplasma mycoides subsp. capri GM12 to get insight into the role of candidate factors constituting the pathways. As expected, glycerol metabolism in GM12 is associated with the highest H 2 O 2 production compared with glycerophosphocholine. The absence of either L-alpha-glycerophosphate oxidase (GlpO) or the glycerol uptake facilitator GlpF, which is the main glycerol importer in GM12, ceased H 2 O 2 production. However, our data did not support any role for the presumed glycerol transport system, GtsABC in glycerol- or glycerophosphocholine-dependent H 2 O 2 production. Caprine peripheral blood mononuclear cells (PBMCs) did not show increased cytotoxic effects upon incubation with GM12 in the presence of glycerol, which does not support H 2 O 2 to be a primary pathogenicity mechanism in GM12.

Source: iScience

DOI: 10.1016/j.isci.2026.117211